Coding

Part:BBa_K2928003:Design

Designed by: Genta Firth   Group: iGEM19_ASIJ_Tokyo   (2019-10-10)


Modified 0.19 Wheat Albumin sequence (Ile to Asn), an α-Amylase inhibitor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Many hours were spent modelling the best amino acids substitutions. We took into account the polarity and charge of the amino acids to strengthen the bonds.


Source

The original 0.19 Wheat albumin sequence was obtained from PDB. Pymol and INTAA Server to model and map the amino acid sequence, to find the most ideal amino acid changes.

References